EGFP expression in the forebrain (olfactory), spinal cord (roof plate, ventral neurones), swimbladder, ear, lateral line, head neural crest (?) and heart.

Genomic location: Chromosome 4

The insertion is in the intron of celsr1a (cadherin EGF LAG seven-pass G-type receptor 1a)

DNA sequences flanking Tol2 insertion site

3:GTTATCTTCATCTTTTTCTGTATAAACCAAACAAGAGCTCATCATCCTCT

TGAAAATTAGTCTTCTGCAATTATTTGTAATTACACTTCACATTCCCTCAGA

ACCAACACCTG